The 5S ribosomal RNA gene is linked to large and small subunit ribosomal RNA genes in the oomycetes, Phytophthora vignae, P. cinnamomi, P. megasperma f.sp. glycinea and Saprolegnia ferax.
Identifieur interne : 002C66 ( Main/Exploration ); précédent : 002C65; suivant : 002C67The 5S ribosomal RNA gene is linked to large and small subunit ribosomal RNA genes in the oomycetes, Phytophthora vignae, P. cinnamomi, P. megasperma f.sp. glycinea and Saprolegnia ferax.
Auteurs : B J Howlett [Australie] ; A G Brownlee ; D I Guest ; G J Adcock ; G I McfaddenSource :
- Current genetics [ 0172-8083 ] ; 1992.
Descripteurs français
- KwdFr :
- ADN ribosomique (génétique), ARN fongique (génétique), ARN ribosomique (génétique), ARN ribosomique 5S (génétique), Données de séquences moléculaires (MeSH), Gènes fongiques (MeSH), Oligonucléotides (MeSH), Oomycetes (génétique), Phytophthora (génétique), Réaction de polymérisation en chaîne (MeSH), Séquence nucléotidique (MeSH), Technique de Southern (MeSH).
- MESH :
English descriptors
- KwdEn :
- Base Sequence (MeSH), Blotting, Southern (MeSH), DNA, Ribosomal (genetics), Genes, Fungal (MeSH), Molecular Sequence Data (MeSH), Oligonucleotides (MeSH), Oomycetes (genetics), Phytophthora (genetics), Polymerase Chain Reaction (MeSH), RNA, Fungal (genetics), RNA, Ribosomal (genetics), RNA, Ribosomal, 5S (genetics).
- MESH :
- chemical , genetics : DNA, Ribosomal, RNA, Fungal, RNA, Ribosomal, RNA, Ribosomal, 5S.
- genetics : Oomycetes, Phytophthora.
- Base Sequence, Blotting, Southern, Genes, Fungal, Molecular Sequence Data, Oligonucleotides, Polymerase Chain Reaction.
Abstract
Southern hybridization and polymerase chain reaction data indicate that the 5S ribosomal RNA gene is linked to the ribosomal RNA gene repeat unit in the oomycetes, Phytophthora vignae, P. cinnamomi, P. megasperma f.sp. glycinea and Saprolegnia ferax, and is apparently transcribed in the same direction as the large and small subunit ribosomal RNA genes. The polymerase chain reaction has been used to amplify all components of the entire ribosomal RNA gene repeat unit for each of these oomycetes. The total size of all amplified products is identical to the size of the ribosomal RNA gene repeat unit, as determined by Southern analysis.
DOI: 10.1007/BF00326410
PubMed: 1473177
Affiliations:
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Le document en format XML
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Brownlee</name></author><author><name sortKey="Guest, D I" sort="Guest, D I" uniqKey="Guest D" first="D I" last="Guest">D I Guest</name></author><author><name sortKey="Adcock, G J" sort="Adcock, G J" uniqKey="Adcock G" first="G J" last="Adcock">G J Adcock</name></author><author><name sortKey="Mcfadden, G I" sort="Mcfadden, G I" uniqKey="Mcfadden G" first="G I" last="Mcfadden">G I Mcfadden</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1473177</idno><idno type="pmid">1473177</idno><idno type="doi">10.1007/BF00326410</idno><idno type="wicri:Area/Main/Corpus">002C64</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002C64</idno><idno type="wicri:Area/Main/Curation">002C64</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002C64</idno><idno 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first="A G" last="Brownlee">A G Brownlee</name></author><author><name sortKey="Guest, D I" sort="Guest, D I" uniqKey="Guest D" first="D I" last="Guest">D I Guest</name></author><author><name sortKey="Adcock, G J" sort="Adcock, G J" uniqKey="Adcock G" first="G J" last="Adcock">G J Adcock</name></author><author><name sortKey="Mcfadden, G I" sort="Mcfadden, G I" uniqKey="Mcfadden G" first="G I" last="Mcfadden">G I Mcfadden</name></author></analytic><series><title level="j">Current genetics</title><idno type="ISSN">0172-8083</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Blotting, Southern (MeSH)</term><term>DNA, Ribosomal (genetics)</term><term>Genes, Fungal (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Oligonucleotides (MeSH)</term><term>Oomycetes (genetics)</term><term>Phytophthora (genetics)</term><term>Polymerase Chain Reaction (MeSH)</term><term>RNA, Fungal (genetics)</term><term>RNA, Ribosomal (genetics)</term><term>RNA, Ribosomal, 5S (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ribosomique (génétique)</term><term>ARN fongique (génétique)</term><term>ARN ribosomique (génétique)</term><term>ARN ribosomique 5S (génétique)</term><term>Données de séquences moléculaires (MeSH)</term><term>Gènes fongiques (MeSH)</term><term>Oligonucléotides (MeSH)</term><term>Oomycetes (génétique)</term><term>Phytophthora (génétique)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Technique de Southern (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Ribosomal</term><term>RNA, Fungal</term><term>RNA, Ribosomal</term><term>RNA, Ribosomal, 5S</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Oomycetes</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN ribosomique</term><term>ARN fongique</term><term>ARN ribosomique</term><term>ARN ribosomique 5S</term><term>Oomycetes</term><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Blotting, Southern</term><term>Genes, Fungal</term><term>Molecular Sequence Data</term><term>Oligonucleotides</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Gènes fongiques</term><term>Oligonucléotides</term><term>Réaction de polymérisation en chaîne</term><term>Séquence nucléotidique</term><term>Technique de Southern</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Southern hybridization and polymerase chain reaction data indicate that the 5S ribosomal RNA gene is linked to the ribosomal RNA gene repeat unit in the oomycetes, Phytophthora vignae, P. cinnamomi, P. megasperma f.sp. glycinea and Saprolegnia ferax, and is apparently transcribed in the same direction as the large and small subunit ribosomal RNA genes. The polymerase chain reaction has been used to amplify all components of the entire ribosomal RNA gene repeat unit for each of these oomycetes. The total size of all amplified products is identical to the size of the ribosomal RNA gene repeat unit, as determined by Southern analysis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1473177</PMID><DateCompleted><Year>1993</Year><Month>02</Month><Day>02</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0172-8083</ISSN><JournalIssue CitedMedium="Print"><Volume>22</Volume><Issue>6</Issue><PubDate><Year>1992</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Current genetics</Title><ISOAbbreviation>Curr Genet</ISOAbbreviation></Journal><ArticleTitle>The 5S ribosomal RNA gene is linked to large and small subunit ribosomal RNA genes in the oomycetes, Phytophthora vignae, P. cinnamomi, P. megasperma f.sp. glycinea and Saprolegnia ferax.</ArticleTitle><Pagination><MedlinePgn>455-61</MedlinePgn></Pagination><Abstract><AbstractText>Southern hybridization and polymerase chain reaction data indicate that the 5S ribosomal RNA gene is linked to the ribosomal RNA gene repeat unit in the oomycetes, Phytophthora vignae, P. cinnamomi, P. megasperma f.sp. glycinea and Saprolegnia ferax, and is apparently transcribed in the same direction as the large and small subunit ribosomal RNA genes. The polymerase chain reaction has been used to amplify all components of the entire ribosomal RNA gene repeat unit for each of these oomycetes. 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